New Releases by Tyler Hamilton

The transmembrane photosynthetic protein complex Photosystem I (PSI) possesses remarkable photoactive electrochemical properties which make it highly sought after for incorporation into biohybrid photovoltaic devices. In the pursuit of all such endeavors, three main factors must always be kept in view: understanding the direct redox transfer steps, threedimensional coordination and stabilization of PSI aggregates, and interfacial connectivity with conductive pathways. This thesis presents steps taken to further these three concepts, contributing to the research community what may help utilize the great potential of PSI. First is presented detailed electrochemical investigations into the role of dissolved O2 as a catalyst for methyl viologen (MV) to scavenge photoactivated electrons from PSI monolayers. These measurements, apart from demonstrating the ability of dissolved O2 in the electrolyte medium to act as a direct electron scavenger, also reveal that the dissolved O2 forms a complex intermediate species with MV which plays the essential role in mediating redox pathways for unidirectional electron transfer processes. Second, a novel 3D architecture is described to organize and stabilize PSI in the myriad of harsh conditions in which it needs to function. PSI is encapsulated in a highly stable nanoporous metal-organic framework, ZIF-8, denoted here as PSI@ZIF-8. The ZIF-8 framework provides a unique scaffold with a robust confining environment for PSI while protecting its precisely coordinated chlorophyll networks from denaturing agents. Pump-probe spectroscopy confirms the photoactivity of the PSI@ZIF-8 composites even after exposure to denaturing agents and organic solvents. This work provides greater fundamental understanding of confinement effects on pigment networks, while significantly broadening the potential working environments for PSIintegrated bio-hybrid materials. Finally, inspired by our successful encapsulation of PSI@ZIF-8, cations from this precursor are used to form charge transfer complexes with the extremely strong organic electron acceptor TCNQ. This PSI-Zn-H2mim-TCNQ charge transfer salt complex was dropcast on ITO as dense films. Through voltammetric cycling and the exchange of Zn2+ and H2mim+ cations, electrochemical annealing of the film increases electrical connectivity and electron conductivity that enabled PSI embedded charge transfer film to generate an order of magnitude greater photocurrents.

Este é um olhar profundo sobre o mundo do ciclismo profissional - e dos problemas de doping que cercam esse esporte e seu mais proeminente corredor, Lance Armstrong. O livro nos leva a fundo a um mundo obscuro, fascinante e surreal de médicos inescrupulosos, diretores de equipe sem limites, e atletas tão cegos pelo sucesso que fariam qualquer coisa para quebrar os limites necessários para vencer. O autor Tyler Hamilton já foi o mais fiel escudeiro de Lance, sempre limpando o caminho do amigo, até ser julgado culpado por doping e banido do esporte, quando conquistou a medalha de ouro nas Olimpíadas. Pela primeira vez, Hamilton expõe sua batalha em clínicas psiquiátricas, fala abertamente sobre as escolhas que tomou para conseguir competir a níveis internacionais, e relata a história de seu complicado relacionamento com Lance Armstrong.

Ranchers, farmers, land managers, and resource management agencies have been plagued with various types of invasive weeds for decades, ultimately affecting millions of acres of viable wildlife habitat, grazing, and recreational opportunities. Invasive weeds often have nutritional contexts that negatively affect animals in some way, increasing the incentive to avoid consuming it by various animal species and domestic livestock. With the current amount of land infested with this weed, and the limited knowledge of the reason for avoidance, the underlying causes of livestock avoidance require in-depth and fundamental understanding. In my MS program, I sought to explore the relationship of plant and herbivore interactions in regards to grazing, animal diet selection through the use of supplementation, along with understanding the chemical compounds that cause avoidance of medusahead by domestic animals. This research will help us better understand the reasoning behind grazing avoidance and preference, and provide further understanding and management approaches using livestock as an alternative management tool in hopes of creating sustainable ecosystems with complementary benefits for soils, plants, animals, and land managers.

Un témoignage unique, d''une franchise exceptionnelle, sur le monde du cyclisme, le système Lance Armstrong et la pratique du dopage à tous les niveaux. Un témoignage unique, d''une franchise exceptionnelle, sur le monde du cyclisme, le système Lance Armstrong et la pratique du dopage à tous les niveaux. " Un jour je suis un type ordinaire, avec une vie normale. Le lendemain je me retrouve sur un trottoir de Madrid avec un téléphone secret et un trou dans le bras qui dégouline de sang, à espérer que je ne me ferai pas prendre. " Tyler Hamilton n''est pas un saint. Il a fait partie des meilleurs et des plus célèbres coureurs cyclistes du monde et remporté une médaille d''or Olympique avant d''être banni de son sport pour dopage en 2009. Il a été le premier lieutenant de Lance Armstrong au sein de l''équipe US Postal entre 1998 et 2001, puis son rival à la tête des équipes CSC et Phonak. Avant sa dégringolade, c''était un héros ordinaire sorti tout droit d''un film des années 50 : jamais un mot plus haut que l''autre, avenant, poli, l''inverse d''une superstar. Bosseur infatigable, dur à la douleur, il s''était patiemment hissé tout en haut de l''échelle. Or cette success-story était une supercherie : dès sa première participation au Tour de France, Hamilton avait compris que pour gagner, il fallait tricher. Le boy scout menait en fait une double vie digne d''un roman d''espionnage : noms de code, téléphones secrets, versements en espèces, et un congélateur médical baptisé " Sibérie " où était entreposé le sang qui servirait pendant le Tour de France. Devenu le confident et l''ami de Lance Armstrong, il s''est retrouvé au cœur du système mis en place par celui-ci pour se doper et gagner, sans se faire prendre.

Glem alt du trodde du visste om doping i Tour de France. Sannheten er mye verre. Tyler Hamilton var en av Lance Armstrongs viktigste hjelperyttere i U.S. Postal. Dette er hans opprivende historie om hvordan dopingen betydde alt i verdens tøffeste sykkelfelt. Kun i ebokutgaven! Les bonuskapittelet som Tyler Hamilton skrev etter at Lance Armstrong bekjente sine synder hos Opera – om Hamiltons latterbrøl, en liten glede og om snøskred. Og Tylers aller siste erkjennelse.

Tyler Hamilton was een van de meest geliefde en snelste wielrenners ter wereld. Zijn jarenlange dopinggebruik heeft hij altijd ontkend. In De wielermaffia vertelt hij voor het eerst zijn verhaal. Hij laat zien hoe de wereld van het professionele wielrennen met de komst van het `wondermiddel EPO midden jaren negentig in korte tijd drastisch veranderde. Om in de Tour met de besten mee te kunnen komen, kon je niet anders dan doping gebruiken. Meer nog dan Hamilton was zijn vriend en ploeggenoot Lance Armstrong een spin in het dopingweb. Hij was het die EPO faciliteerde, contacten onderhield met de meest vooraanstaande artsen onder wie de sluwe Italiaanse dokter Michele Ferrari en zijn mederenners dwong om doping te gebruiken.

“The holy grail for disillusioned cycling fans . . . The book’s power is in the collective details, all strung together in a story that is told with such clear-eyed conviction that you never doubt its veracity. . . . The Secret Race isn’t just a game changer for the Lance Armstrong myth. It’s the game ender.”—Outside NEW YORK TIMES BESTSELLER • WINNER OF THE WILLIAM HILL SPORTS BOOK OF THE YEAR AWARD The Secret Race is the book that rocked the world of professional cycling—and exposed, at long last, the doping culture surrounding the sport and its most iconic rider, Lance Armstrong. Former Olympic gold medalist Tyler Hamilton was once one of the world’s top-ranked cyclists—and a member of Lance Armstrong’s inner circle. Over the course of two years, New York Times bestselling author Daniel Coyle conducted more than two hundred hours of interviews with Hamilton and spoke with numerous teammates, rivals, and friends. The result is an explosive page-turner of a book that takes us deep inside a shadowy, fascinating, and surreal world of unscrupulous doctors, anything-goes team directors, and athletes so relentlessly driven to win that they would do almost anything to gain an edge. For the first time, Hamilton recounts his own battle with depression and tells the story of his complicated relationship with Lance Armstrong. This edition features a new Afterword, in which the authors reflect on the developments within the sport, and involving Armstrong, over the past year. The Secret Race is a courageous, groundbreaking act of witness from a man who is as determined to reveal the hard truth about his sport as he once was to win the Tour de France. With a new Afterword by the authors. “Loaded with bombshells and revelations.”—VeloNews “[An] often harrowing story . . . the broadest, most accessible look at cycling’s drug problems to date.”—The New York Times “ ‘If I cheated, how did I get away with it?’ That question, posed to SI by Lance Armstrong five years ago, has never been answered more definitively than it is in Tyler Hamilton’s new book.”—Sports Illustrated “Explosive.”—The Daily Telegraph (London)

An “illuminating and important” look at the scientists, engineers, and entrepreneurs who are working to save us from catastrophic climate change (New York Journal of Books). Nikola Tesla was considered a mad scientist by the society of his time for predicting global warming more than a hundred years ago. Today, we need visionaries like him to find sources of alternative energy and solutions to this looming threat. Mad Like Tesla takes an in-depth look at climate issues, introducing thinkers and inventors such as Louis Michaud, a retired refinery engineer who claims we can harness the energy of man-made tornadoes, and a professor and a businessman who are running a company that genetically modifies algae so it can secrete ethanol naturally. These individuals and their unorthodox methods are profiled through first-person interviews, exploring the social, economic, financial, and personal obstacles that they continue to face. Also covered is the existing state of green energy technologies—such as solar, wind, biofuels, smart grid, and energy storage—offering a ray of hope against a backdrop of dread. “Hamilton makes complex technologies comprehensible.” —Library Journal

Canadian thought leaders who participated in this CEGN exercise identified a number of opportunities associated with the green economy. However, they were not blind to the many obstacles that must be overcome before the envisioned benefits can be delivered. They saw tremendous potential for green job creation, but also for long-term economic growth as a result of enhanced productivity and global competiveness. They appreciated that the greening of Canada''s economy would mean more sustainable management of the country''s natural resources and a cleaner, healthier environment for generations of future Canadians. Canada would also be seen as doing its role to stabilize human-caused climate change. The effort - the process of greening the economy - would also bring healthy returns on investment to the financial community. The local nature of many green initiatives would bring the country''s communities and First Nations groups closer together by encouraging greater collaboration and partnering at the local level.

Evenness was higher in susceptible watersheds, which were distinct in species composition. Riparian and upland habitats in susceptible watersheds supported complementary small mammal communities, while communities in the non-susceptible watershed were more homogenous. Susceptible watersheds are located at the lowest elevations of GBNP where habitat heterogeneity due to the contrast between mesic riparian and xeric upland habitats is important in maintaining small mammal diversity.

Abstract: The presence of 3-nitrotyrosine (nTyr) in proteins and peptides serves as a marker for the production of reactive nitrogen species. Nitrotyrosine occurs in cells in vivo at normal, basal levels, and in increasing amounts during states involving oxidant stress, such as inflammation. Tyrosine nitration can change the activity of many enzymes and is believed to occur in a site-specific manner. Tandem mass spectrometry provides the definitive method for identifying the presence and site of nitration, but the amounts of nTyr in vivo are often below detectable levels. To address this need we developed methods to specifically concentrate proteins and peptides containing nTyr to facilitate the identification of the site of modification. The first series of methods focused on specific, solution-phase labeling of dithionite-reduced nitrotyrosine with an amine-reactive biotin label (sulfo-NHS-S-S-biotin). After protein digestion, biotinylated peptides were affinity-captured with streptavidin-bound Sepharose and eluted by reducing the disulfide linker on the biotin tag. Although nitrated peptides from tetranitromethane-treated bovine serum albumin could be isolated with several variants of the method, all required very large amounts of sample to yield suitable data. A solid-phase capture approach was developed that used similar chemistry and also took advantage of the lower pKa of aminotyrosine to increase selectivity of binding. This yielded a 10-fold increase in sensitivity. The best implementation of the method, however still suffered from inadequate sensitivity and reproducibility. Of the two methods presented, however, it holds greater potential. Given the amount of non-specific reactivity of amines with the matrices that were used, it may he necessary to develop a more specific capture chemistry. The substrates also may have difficulty accessing reactive sites on the resin. Further optimization of binding conditions might yield a more robust and reproducible capture reaction. Given the methods developed, and with potential improvements to the reaction conditions, solid-phase capture appears to be a promising method to isolate nTyr-containing peptides and proteins.